Site ID:
BR-BIO
Site Name:
Manaus - ZF2 BIONTE
Variables:
Air Temperature; Leaf photosynthetic traits (Vcmax and Jmax); Leaf spectra; Leaf temperature; Leaf water potential; Solar Radiation; Stomatal conductance; Wind speed/direction
Date Range:
May 17, 2023
-
Nov. 2, 2023
Description:
Leaf traits measured at the top of the canopy on 30 tree species in the BIONTE experimental forest, near Manaus, Brazil, at three time points during August to November, 2023. The aim of this study was to test the relationship between leaf water use efficiency and wood density. Leaf gas exchange was measured on top-of-canopy leaves accessed by an articulated boom lift. Response curves (A-Ci and A-Q), and dark adapted respiration were measured on cut branches. Survey gas exchange measurements were performed on attached leaves at various times during the day. Following gas exchange, leaves were harvested, and measured for leaf water potential and spectral reflectance. Data in this data package is presented in csv files, with metadata in csv and descriptive experimental protocols as pdf. Gas exchange data and metadata meets the requirements of the ESS-DIVE reporting format for leaf-level gas exchange data and metadata.
QA/QC:
Full QA-QC
Methods Description:
Leaf-level gas exchange measurements were made on top-of-canopy, sunlit leaves from 30 tree species using four LI-COR 6400 XT photosynthesis systems (LI-COR Biosciences, Lincoln NE, USA). A Genie Z 80-60 articulated boom lift was used to access the canopy top. On each day of measurements, branches were cut pre-dawn or around dawn, recut underwater to restore hydraulic conductivity, and kept in buckets in a shaded area. Traditional photosynthetic response curves to CO2 (A-Ci), light (A-Q), and dark-adapted respiration (Rdark) were measured for each species. A series of steady-state gas exchange measurements were also made on attached top-of-canopy leaves, taking sufficient time for photosynthesis and stomata to acclimate (> 20 minutes). These were made at light levels of 1800, 400 or 0 micromol m-2 s-1. Air temperature, wind speed, incident light and leaf temperature were recorded to accompany each of these survey measurements.
After completion of gas exchange measurements, the leaf water potential and leaf spectral reflectance were recorded for each leaf sample. Leaf water potential (LWP) was measured using a PMS Model 1000 Scholander-type pressure chamber, using standard practices. Full spectrum (350 – 2500 nm) leaf reflectance was measured on detached leaves using an ASD FieldSpec 3 (ASD Inc, Boulder CO, USA). On a number of days prior to the gas exchange campaigns LWP was also measured on samples without associated gas exchange or spectral measurements. See the included protocol documents for a complete description of Methods. Data have undergone complete QAQC and are formatted to follow ESS-DIVE Leaf-level gas exchange, CSV file and File level metadata reporting formats.
Access Level:
Public
Originating Institution(s):
Brookhaven National Laboratory; Instituto Nacional de Pesquisas da Amazônia (INPA); Centre de Recherche sur la Biodiversité et l'Environnement (CRBE)
Sponsor Organization(s):
None
Contact:
Rogers, Alistair - Brookhaven National Laboratory (arogers@bnl.gov)
Version:
1.0
Dataset Citation:
Lamour J; Souza D; Gimenez B; Oliveira R; Ely K; Plácido A; Menezes V; Rogers A (2024): Leaf gas exchange, leaf water potential and spectral reflectance, BIONTE, Brazil, 2023. 1.0. NGEE Tropics Data Collection. (dataset). https://doi.org/10.15486/ngt/2337880
Acknowledgement:
This research was supported as part of NGEE-Tropics, funded by the U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research under contract no. DE-SC0012704
Data Link: Download Dataset
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